Analysis Note

Positive ControlRecombinant G-CSF protein, bone marrow tissue, or fetal liver tissue

Application

Immunoblotting (1-5 µg/ml)r>Immunofluorescence (1-5 µg/ml)r>Paraffin Sections (1-10 µg/ml, saponin pre-treatment required, see comments)

General description

Recognizes the ~19 kDa G-CSF glycoprotein in bone marrow and fetal liver tissue.

Anti-Granulocyte Colony Stimulating Factor (Ab-1) mouse monoclonal, clone 5.24, recognizes ~19 kDa G-CSF in bone marrow & fetal liver tissue. It is validated for use in WB, IF & IHC (paraffin).

Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes granulocyte colony stimulating factor.

Immunogen

Human

purified human G-CSF

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Nicola, N.A. 1987. J. Cell Cloning5, 1.Metcalf, D., 1986. Blood67, 257.Nagata, S., et al. 1986. Nature319, 415.Nagata, S., et al. 1986 EMBO J.5, 575.Souza, L.M., et al. 1986 Science232, 61.Metcalf, D. 1984, Hemopoietic colony stimulating factors. Amsterdam: Elsevier.Nicola, N.A. Vadas, M.A., 1984. Immunol Today5, 76.Nicola, N.A., et al. 1983 J. Biol. Chem.258, 9017.Burgess, A.W., Metcalf, D. 1980. J. Cancer26, 647.

For paraffin sections, saponin treatment is required for antigen retrieval. Antibody should be titrated for optimal results in individual systems.

Packaging

200 µg in Glass bottle

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Warning

Toxicity: Standard Handling (A)